How Are Ewing Tumors Diagnosed?
Ewing tumors are usually found because of signs or symptoms a child or teen is having. If a tumor is suspected, tests will be needed to find out for sure.
Medical history and physical exam
If your child has signs or symptoms that could be from a tumor, the doctor will want to get a complete medical history to find out more about the symptoms and how long they have been present. The doctor will also do a complete physical exam, paying special attention to any areas causing pain or swelling.
If a doctor suspects the child might have a bone tumor (or another type of tumor), more tests will be done to find out. These might include imaging tests, biopsies, and/or lab tests.
Imaging tests (such as x-rays, MRI scans, CT scans, bone scans, and PET scans) create pictures of the inside of the body. Imaging tests can be done for many reasons, including:
- To help find out if a suspicious area might be cancer
- To determine the extent of a tumor or learn how far a cancer may have spread
- To help determine if treatment is working
Patients who have or may have a Ewing tumor will have one or more of these tests.
If a bone lump doesn’t go away or the doctor suspects a bone tumor for some other reason, an x-ray of the area will probably be the first test done. A radiologist (doctor who specializes in reading imaging tests) can usually spot a bone tumor on an x-ray and can often tell if it is likely to be a Ewing tumor. But other imaging tests may be needed as well.
Even if an x-ray strongly suggests a Ewing bone tumor, a biopsy (described below) is still needed to confirm that it is cancer rather than some other problem, such as an infection.
Magnetic resonance imaging (MRI) scan
Often, an MRI scan is done to get a better look an abnormal area seen on an x-ray. MRI scans usually can show if it is likely to be a tumor, an infection, or some type of bone damage from other causes. MRI scans can also help determine the extent of a tumor, as they show in detail the marrow inside bones and the muscle, fat, and connective tissue around the tumor. Knowing the extent of the tumor is very important when planning surgery or radiation therapy.
MRI scans might also be done to see if the cancer has spread to other areas, such as the spine or pelvis (hip area). MRI scans can also be used during and after treatment to see how well the tumor is responding.
MRI scans create detailed images using radio waves and strong magnets instead of x-rays, so there is no radiation involved. A contrast material called gadolinium may be injected into a vein before the scan to help see details better.
MRI scans may take up to an hour. Your child may have to lie on a table that slides inside a narrow tube, which is confining and can be distressing. The test also requires a person to stay still for several minutes at a time. Open MRI machines, which are less confining, might be another option, but they still require staying still for long periods of time. The machines also make buzzing and clicking noises that may be disturbing. Sometimes, younger children are given medicine to help keep them calm or even asleep during the test.
Computed tomography (CT or CAT) scan
CT scans of the chest are often used to see if a Ewing tumor has spread to the lungs. MRI scans are usually a bit better at defining the extent of the main tumor itself, but a CT scan of the tumor may be done as well.
The CT scan uses x-rays to make detailed cross-sectional images of parts of the body, including soft tissues such as muscles. Instead of taking one picture, like a regular x-ray, a CT scanner takes many pictures as it rotates around your child while he or she lies on a table. A computer then combines these pictures into images of slices of the part of the body being studied.
Before the scan, your child may be asked to drink a contrast solution and/or get an intravenous (IV) injection of a contrast dye that helps better outline abnormal areas in the body. If a contrast dye is to be injected, your child may need an IV line. The contrast can cause some flushing (a feeling of warmth, especially in the face). Some people are allergic and get hives. Rarely, more serious reactions like trouble breathing or low blood pressure can occur. Be sure to tell the doctor if your child has any allergies (especially to iodine or shellfish) or has ever had a reaction to any contrast material used for x-rays.
CT scans take longer than regular x-rays, but not as long as MRI scans. A CT scanner has been described as a large donut, with a narrow table that slides in and out of the middle opening. Your child will need to lie still on the table while the scan is being done. Some people feel a bit confined while the pictures are being taken, although it is not as narrow as an MRI tube. Some children may need to be sedated before the test to stay still and help make sure the pictures come out well.
A bone scan can help show if a cancer has metastasized (spread) to bones in other parts of the body, and might be part of the workup for a child with a Ewing tumor. This test is useful because it can show the entire skeleton at once. (A positron emission tomography [PET] scan can often provide similar information, so a bone scan might not be needed if a PET scan is done.)
For this test, a small amount of low-level radioactive material is injected into a vein (intravenously, or IV). (The amount of radioactivity used is very low and will pass out of the body within a day or so.) The substance settles in abnormal areas of bone throughout the body over the course of a couple of hours. Your child then lies on a table for about 30 minutes while a special camera detects the radioactivity and creates a picture of the skeleton. Younger children might be given medicine to help keep them calm or even asleep during the test.
Areas of active bone changes appear as “hot spots” on the skeleton because they attract the radioactivity. These areas may suggest the presence of cancer, but other bone diseases can also cause the same pattern. To be sure, other tests such as plain x-rays or MRI scans, or even a bone biopsy might be needed.
Positron emission tomography (PET) scan
For a PET scan, a form of radioactive sugar (known as fluorodeoxyglucose or FDG) is injected into the blood. The amount of radioactivity used is very low and will pass out of the body within a day or so. Because cancer cells in the body are growing quickly, they absorb large amounts of the sugar. After about an hour, your child will lie on a table in the PET scanner for about 30 minutes while a special camera creates a picture of areas of radioactivity in the body. The picture is not detailed like a CT or MRI scan, but it provides helpful information about the whole body.
PET scans can be very helpful in showing the spread of Ewing tumors and in finding out whether abnormal areas seen on other imaging tests (such as a bone scan or CT scan) are tumors. PET scans can also be repeated during treatment to monitor the cancer over time.
Some newer machines can do a PET and CT scan at the same time (PET/CT scan). This lets the doctor compare areas of higher radioactivity on the PET scan with the more detailed appearance of that area on the CT scan.
To learn more about these and other imaging tests, see our document Imaging (Radiology) Tests.
Biopsy of the tumor
The results of imaging tests might strongly suggest a Ewing tumor, but a biopsy (removing some of the tumor for viewing under a microscope and other lab testing) is the only way to be certain. A biopsy is also the best way to tell Ewing tumors from other types of cancer.
If the tumor is in a bone, it is very important that a surgeon experienced in treating bone tumors does the biopsy. Whenever possible, the biopsy and the surgery to treat the cancer should be planned together, and the same surgeon should do both. Proper planning of the biopsy can help prevent later complications and might reduce the amount of surgery needed later on.
There are a few ways to get a sample of the tumor to diagnose Ewing tumors.
In very rare cases, if the tumor is small enough and in a good location, the surgeon can completely remove it while the child is under general anesthesia (asleep). This is called an excisional biopsy.
In most cases of suspected Ewing tumors, an incisional biopsy (taking only a piece of the tumor) is more likely to be done. This can be done in a couple of ways:
- Surgical (open) biopsy: For this type of biopsy, the surgeon cuts away a piece of the tumor through an opening on the skin.
- Needle (closed) biopsy: In this type of biopsy, the surgeon puts a large, hollow needle through the skin and into the tumor to remove a piece of it.
Incisional biopsies are often done while the patient is under general anesthesia (in a deep sleep), but in older teens and adults they are sometimes done using sedation and a local anesthetic (numbing medicine).
If general anesthesia is going to be used for the biopsy, the surgeon may also plan other procedures while the child is asleep to avoid having to do them as separate operations later on. For example, if the tumor is thought to have spread to the chest or elsewhere, the surgeon may take biopsy samples of these suspected tumors when the child is still asleep. The doctor might also do a bone marrow biopsy (see next section) at this time to see if the cancer has spread to the bone marrow spaces.
During the biopsy (while the child is still asleep), a pathologist (a doctor specializing in lab tests to diagnose diseases) can take a quick look at the biopsy samples under a microscope. If it looks like a Ewing tumor, the child will very likely need chemotherapy as part of treatment, so the surgeon may place a small flexible tube, known as a central venous catheter, into a large vein in the chest area during the same operation. The catheter end lies just under or outside on the skin. It can stay in place for several months during treatment. The catheter gives doctors and nurses easier access to the vein, which is allows the child to get fewer needle sticks when chemotherapy is given or blood needs to be drawn at a later time.
Bone marrow aspiration and biopsy
These tests are used to see if the cancer cells have spread into the bone marrow, the soft inner parts of certain bones. The tests aren’t usually done to diagnose Ewing tumors, but they may be done once the diagnosis is made because it is important to know if the tumor has spread to the bone marrow.
Bone marrow aspiration and biopsy are usually done at the same time. In most cases the marrow samples are taken from the back of both of the pelvic (hip) bones.
These tests may be done during the surgery to biopsy or treat the main tumor (while the child is still under anesthesia), or they may be done as a separate procedure.
If the bone marrow aspiration is being done as a separate procedure, the child lies on a table (on his or her side or belly). The area over the hip is cleaned, and the skin and the surface of the bone are numbed with a local anesthetic, which may cause a brief stinging or burning sensation. In most cases, the child is also given other medicines to make them sleepy, or they might even be asleep during the procedure. A thin, hollow needle is then inserted into the bone, and a syringe is used to suck out a small amount of liquid bone marrow.
A bone marrow biopsy is usually done just after the aspiration. A small piece of bone and marrow is removed with a slightly larger needle that is pushed down into the bone. Once the biopsy is done, pressure is applied to the site to help stop any bleeding.
Samples from the bone marrow are sent to a pathology lab, where they are looked at and tested for cancer cells.
Testing biopsy samples
A doctor called a pathologist looks at all biopsy specimens under a microscope to see if they contain cancer cells. If cancer is found, the specific type of cancer can often be determined as well. But because cells from Ewing tumors share many of the same features as cells from other types of childhood cancer, more lab tests are often needed.
For this test, a portion of the biopsy sample is treated with special proteins (antibodies) that attach to substances found on Ewing tumor cells but not on other cancers. Chemicals (stains) are then added so that cells containing these substances change color and can be seen under a microscope. This lets the pathologist know that the cells are from a Ewing tumor.
For this test, chromosomes (pieces of DNA) from the tumor cells are looked at under a microscope to detect any changes. Ewing tumor cells almost always have chromosome translocations, where 2 chromosomes swap pieces of their DNA. In most cases, the cells have translocations between chromosomes 22 and 11. Less often, the translocation is between other chromosomes. Finding these changes can help doctors tell Ewing tumors from other types of cancer. Other types of chromosome changes can also be found in some Ewing tumors.
Getting the results of cytogenetic testing usually takes about 2 to 3 weeks because the cancer cells must be grown in lab dishes for a couple of weeks before their chromosomes can be seen under the microscope.
Fluorescence in situ hybridization (FISH) is a type of cytogenetic test that uses special fluorescent dyes to spot specific chromosome changes in Ewing tumors. FISH can find most chromosome changes (such as translocations) that are visible under a microscope in standard cytogenetic tests, as well as some changes too small to be seen with usual cytogenetic testing.
FISH can be used to look for specific changes in chromosomes. It is very accurate and can usually provide results within a couple of days.
Reverse transcription polymerase chain reaction (RT-PCR)
This test is another way to find translocations in tumor cells to confirm the type of tumor. RT-PCR is a very sensitive test that is often able to detect very small numbers of cells with translocations, which wouldn’t be detected by cytogenetics.
RT-PCR is also useful in looking for leftover or recurrent cancer after treatment. For example, if RT-PCR testing of a bone marrow sample after treatment finds cells with a typical Ewing tumor translocation, it is likely that the cancer has not been cured, so more treatment is needed.
No blood test can be used to diagnose Ewing tumors. But certain blood tests may be helpful once a diagnosis has been made.
A complete blood count (CBC) measures the levels of white blood cells, red blood cells, and platelets in the blood. An abnormal CBC result at the time of diagnosis might suggest the cancer has spread to the bone marrow, where these blood cells are made.
A blood test for levels of an enzyme called lactate dehydrogenase (LDH) is typically done at diagnosis. A high LDH level indicates the cancer may be harder to treat.
Standard blood tests are done often to check a child’s general health both before treatment (especially before surgery) and during treatment (such as chemotherapy) to look for possible problems or side effects. These tests often include a CBC to monitor bone marrow function and blood chemistry tests to measure how well the liver and kidneys are working.
Last Medical Review: September 18, 2014 Last Revised: February 4, 2016